POPULATION GENETIC STRUCTURE OF SPINY LOBSTERS, Panulirus homarus AND Panulirus ornatus, IN THE INDIAN OCEAN, CORAL TRIANGLE, AND SOUTH CHINA SEA

Populations of spiny lobsters worldwide are threatened by overfishing, while its supply from aquaculture is currently insufficient to meet the market demand. This current study investigated the genetic structure of two economically important spiny lobsters, P. homarus and P. ornatus sourced from the Indian Ocean and South China Sea. Fragments of the cytochrome oxidase subunit-I (COI) gene of the mitochondrial DNA of 71 P. homarus and 42 P. ornatus collected from 6 and 5 fish landing sites in Indonesia, respectively, were sequenced. Homologous sequences from the Indian Ocean and South China Sea available at GenBank were included in the analysis. No genetic differences were observed in P. ornatus populations from the two geographic regions (xST = -0.005) while a diminutive difference was found in the populations of P. homarus (xST = -0.002 and 0.009). These results, combined with a negative Tajima’s D estimates, points to a deficit of nucleotide variation relative to the expectations from the mutation/drift equilibrium. Reconstruction of the phylogeny of P. homarus demonstrates that all Indonesian samples of P. homarus are grouped in one cluster and share the common cluster with GenBank data originated from Taiwan, Vietnam, India, Sri Lanka, Oman, and Iran. The phylogeny of P. ornatus indicates that there are two separated lineages existing in Indonesia

Genomic signatures of host-associated divergence and adaptation in a coral-eating snail, Coralliophila violacea (Kiener, 1836).

The fluid nature of the ocean, combined with planktonic dispersal of marine larvae, lowers physical barriers to gene flow. However, divergence can still occur despite gene flow if strong selection acts on populations occupying different ecological niches. Here, we examined the population genomics of an ectoparasitic snail, Coralliophila violacea (Kiener 1836), that specializes on Porites corals in the Indo-Pacific. Previous genetic analyses revealed two sympatric lineages associated with different coral hosts. In this study, we examined the mechanisms promoting and maintaining the snails' adaptation to their coral hosts. Genome-wide single nucleotide polymorphism (SNP) data from type II restriction site-associated DNA (2b-RAD) sequencing revealed two differentiated clusters of C. violacea that were largely concordant with coral host, consistent with previous genetic results. However, the presence of some admixed genotypes indicates gene flow from one lineage to the other. Combined, these results suggest that differentiation between host-associated lineages of C. violacea is occurring in the face of ongoing gene flow, requiring strong selection. Indeed, 2.7% of all SNP loci were outlier loci (73/2,718), indicative of divergence with gene flow, driven by adaptation of each C. violacea lineage to their specific coral hosts

PENEGUHAN DIAGNOSIS PENYAKIT NEWCASTLE DISEASE LAPANG PADA AYAM BURAS DI BALI MENGGUNAKAN TEKNIK RT-PCR

Penelitian ini bertujuan mendiagnosis kasus newcastle disease (ND) lapangan pada ayam bukan ras yang bersifat akut melalui hasil pemeriksaan di Laboratorium Patologi dan Laboratorium Biomedik Fakultas Kedokteran Hewan Universitas Udayana tahun 2008-2009. Sebanyak sepuluh ekor sampel ayam buras telah diperiksa. Gejala klinis yang teramati meliputi: anoreksia, lesu, bersin, batuk, dan diare putih kehijauan dengan diagnosis sementara sebagai penyakit ND yang bersifat akut. Sampel diambil dari organ yang mengalami perubahan patognomonis seperti pada proventrikulus, ventrikulus, seka tonsil, paru-paru dan otak. Perbanyakan virus menggunakan telur ayam bertunas (TAB) umur 10 hari melalui ruang alantois dan diinkubasikan pada inkubator telur suhu 37° C selama 3 hari. Koleksi cairan alantois dilakukan pada hari ke-3, selanjutnya diidentifikasi dengan uji serologi hemaglutinasi (Haemaglutination-Inhibition Test/HA/HI) dengan teknik mikrotiter baku dan dikonfirmasi dengan uji reverse transcriptase-polymerase chain reaction (RT-PCR) menggunakan primer FNDIFP (5’-CCCCGTTGGAGGCATAC-3’) dan FNDIBP (5’-TGTTGGCAGCATTTTGATTG-3’). Hasil penelitian menunjukkan bahwa semua sampel kasus ayam bukan ras yang diperiksa positif terinfeksi oleh virus penyakit ND akut. Kajian ini mengindikasikan bahwa penyakit ND di Bali masih bersifat endemis

DETEKSI VTRAL NERVOUS ITECROSTS (VNN) PADA BENTH KERAPU MACAN (Epinephelus fuscoguttatus) DENGAN METODE POLYMERASE CHATN REACTTON (PCR)

Penelitian ini dilakukan di Laboratorium Balai Besar Riset Perikanan Budidaya Laut, Gondol-Bali bertujuan untuk mengetahui infeksi virus-VNN pada pemeliharaan benih ikan kerapu macan yang memiliki ukuran berbeda

Outcome following cervicothoracic junction fusion in T1 pathological fracture of breast cancer spinal metastases: a case report

The cervicothoracic junction (CTJ) is defined as the area extending from vertebral segment C7 to T2. Spinal metastases of CTJ are rare, range from 10% to less than 20%. A 47-year-old woman complained sensory and motor disturbance since 3 weeks prior to admission. History of lump on the left breast was confirmed. Neurological deficit was confirmed as ASIA C at the time of diagnosis. MRI finding suggest fracture of T1 vertebral body with kypothic angle 28° that causing anterior compression of spinal cord. The patient underwent decompression and posterior fusion from C4 to T4. A biopsy sample was also collected from the spine and left breast to confirm the diagnosis. Patient evaluation was done during discharge and at certain points of follow-up for improvement on its neurological, pain, and functional status. An MRI evaluation was performed to evaluate spinal stability and fusion. Significant improvements were observed in patient ambulatory and pain status. Cervicothoracic junction fusion procedure is a considerable choice for the management of pathological vertebral fractures with cervicothoracic junction involvement caused by spinal metastases of breast cancer

Medial swivel type dislocation of the talonavicular joint with associated cuboid fracture: a case report

Medial Swivel-type dislocation are dislocation of talonavicular joint (TNJ) medially and the calcaneus swivels under the talus, with the calcaneocuboid joint intact. This is a rare injury due to the strong network of ligament and tendinous structures. An 11-year-old girl presented after 1 week of injury to the left foot. She had pain, swelling on the mid-foot and inability to weight bearing. X-ray and computerized tomography (CT) scan showed medial dislocation of TNJ, with fracture of cuboid body. A closed reduction was attempted but it failed. Patient then underwent open reduction with K-wire fixation and immobilization by below knee cast for 3 weeks. After K-wire removal, the foot was stable with near normal ankle and sub-talar joint range of motion and patient started to partial weight bearing Midtarsal dislocations of the foot are rare injuries. In this case dislocation is resulted from high-energy medial forces to the forefoot. The associated cuboid fracture possibly as a result of tensile forces through the lateral structure of midfoot with adduction. ‘Swivel dislocation’ in which the TNJ dislocates, usually medially, and the calcaneus swivels under the talus, with the calcaneocuboid joint intact. A careful assessment of initial radiograph and CT scan should be done to determine type of dislocation and associated fracture. An open reduction K-wire fixation bring a stable reduction. Talonavicular dislocations are rare injuries, occur as a result of high-energy trauma. A stable reduction and good outcome are anticipated

Optimalisasi Jenis Olahan Kering dan Cara Pengolahan Akhir Ketela

The research was aimed to determine the effect of the form of dry food products, and final processing of sweet potato and their interaction, on the nutrition content, resistant starch of the products, and the increasing level of blood glucose of mice after consuming the products. The method of the research was based on the completed random design with the factorial experiment two factors were experimented, the first factor was the form of dry food products with four kinds of the product from namely: crispy, chip, cracker and snack. The second factor was final processing with two kinds of methods were experiment was done using so nice which divided into eight blocks of combination treatments of the experiments. The crispy form of dry sweet potato product and final processed by oven roasting was the best product which could suppress the increasing level of blood glucose of mice to 8 mg/L and has resistant starch of about 39.29%. This product also had the best nutrition content such as content of protein, lipid, total sugar and starch of about 3.42%, 1.79%, 7.98%, and 50.02%

Identification of Pathogenecity of Avian Influenza Virus Subtype H5N1 from Waterfowls Base on Amino Acid Sequence of Cleavage Site Hemagglutinin Protein

Identification of pathotype of Avian Influenza Virus (AIV) subtype H5N1 isolates is very important. Thisresearch aimed to identify the pathotype of AIV subtype H5N1 isolated from household waterfowls in West Javabased on molecular markers of amino acid sequences of the Hemagglutinin (HA) cleavage site. Fragments of HAgenes of 21 isolates were amplified using RT-PCR with a primer pair that flanking the cleavage site region, andsequenced with dideoxy-termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignmentof nucleotide and their deduced amino acid sequence were analyzed using ClustalW from MEGA 3.1 program.The result shows that all H5N1 isolates (21 isolates) possess polybasic cleavage sites with 2 patterns ofamino acid sequence, i.e QRERRRKKR (20 isolates) and QRESRRKKR (1 isolate). This finding indicates that all ofthe viruses isolated in this research were of highly pathogenic avian influenza (HPAI) strains.Keywords: cleavage site, waterfowls, HPA

REVALENSI PORCINE CIRCO VIRUS SECARA SEROLOGIS PADA PETERNAKAN BABI DI BALI

Penelitian ini bertujuan mengetahui seroepidemiologi infeksi porcine circo virus (PCV-2) dua pada peternakan babi di Bali. Pada penelitian ini sampel yang dianalisis sebanyak 295 sampel. Sampel berasal dari peternakan babi rakyat sebanyak 98 dan dari peternakan babi intensif sebanyak 197. Sampel berasal dari delapan kabupaten dari sembilan kabupaten yang ada di Bali. Deteksi antibodi dilaksanakan dengan uji enzyme-linked immunosorbent assay (ELISA) dan deteksi virus dilakukan dengan polymerase chain reaction (PCR). Hasil penelitian menunjukkan bahwa seroprevalensi antibodi anti-PCV-2 adalah 84,1%, dengan sebaran di peternakan rakyat dan peternakan intensif masingmasing sebesar 70,4 dan 91,2%. Semua peternakan babi intensif menunjukkan antibodi positif. Prevalensi virus PCV-2 di seluruh Bali sebesar 1,7% dengan sebaran pada peternakan rakyat peternakan intensif masing-masing sebesar 3,1 dan 1,0%. Dari hasil penelitian disimpulkan bahwa infeksi PCV-2 pada peternakan babi di Bali bersifat endemis